![]() ![]() Gram Negative Bacteria: The outer layer is absent in gram negative bacteria. Gram Positive Bacteria:The outer membrane is present in gram positive bacteria. Gram Negative Bacteria:Gram negative bacteria appear in pink by retaining the counterstain safranin. Gram Positive Bacteria:Gram positive bacteria appear in purple color under the microscope. Gram Negative Bacteria: Gram negative bacteria do not retain the crystal violet stain during gram staining, giving the negative result. Gram Positive Bacteria:Gram positive bacteria retain the crystal violet stain during gram staining, giving the positive result. The gram staining of rod-shaped Bacillus species is shown in figure 1.įigure 4: Gram Negative Cell Wall Difference Between Gram Positive and Gram Negative Bacteria Gram Staining One of the most characteristic features of gram positive bacteria is that they are more susceptible to antibiotics due to a lack of an outer membrane. The thick peptidoglycan layer present in the gram positive bacteria is responsible for retaining the stain even after decolorization. They appear in purple color under the microscope by staining. What is the difference between Gram Positive and Gram Negative Bacteriaīacteria which retain the crystal violet stain during gram staining, giving the positive color for tests, are called gram positive bacteria. – Definition, Characteristics, Cell Wall and Cell Envelope Structureģ. – Definition, Characteristics, Cell Wall Structure, Examples The main difference between gram positive and gram negative bacteria is that gram positive bacteria contain a thick peptidoglycan cell wall along with teichoic acid, allowing the bacteria to stain in purple during gram staining whereas gram negative bacteria contain a thin peptidoglycan cell wall with no teichoic acid, allowing the cell wall to stain in pink during counter staining. Therefore, gram negative bacteria are more pathogenic compared to gram positive bacteria. Since gram negative bacteria contain an outer membrane, they are less susceptible to antibiotics. Gram positive bacteria are more susceptible to antibiotics due to the lack of an outer membrane. The stain used during the technique is crystal violet. Gram staining was developed by Cristian Gram in 1884. Gram positive and gram negative bacteria are the two types of bacteria, differentiated by the gram staining technique. coli), Salmonella, Shigella, and other Enterobacteriaceae, Pseudomonas, Moraxella, Helicobacter, Stenotrophomonas, Bdellovibrio, acetic acid bacteria, Legionella etc.Main Difference – Gram Positive vs Gram Negative Bacteria Gram negative bacteria: Escherichia coli (E.Gram positive bacteria: Actinomyces, Bacillus, Clostridium, Corynebacterium, Enterococcus, Gardnerella, Lactobacillus, Listeria, Mycoplasma, Nocardia, Staphylococcus, Streptococcus, Streptomyces, etc.Source: Examples of Gram Positive and Gram Negative Bacteria ![]() Gram Negative bacteria: red or pink color.Gram Positive bacteria: blue or purple color.Air dry method can also be used after shaking off excess water The safranin solution is washed off with water, and excess water is blotted with the bibulous paper. The smear is counterstained with safranin solution for 40 to 60 seconds. ![]() To prevent excess decolorization in the gram-positive cells, addition of decolorizer is stopped as soon as the solvent is not colored as it flows over the slide
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